Utility of Bartonella henselae IgM-Western Blot Bands for Serodiagnosis of Cat Scratch Disease.

Utility of Bartonella henselae IgM-Western Blot Bands for Serodiagnosis of Cat Scratch Disease.

J Clin Microbiol. 2017 Nov 01;:

Authors: Otsuyama KI, Tsuneoka H, Yoshidomi H, Haraguchi M, Yanagihara M, Tokuda N, Nojima J, Ichihara K

Abstract
We evaluated the utility of Western blot (WB) bands of Bartonella henselae in detecting anti-B. henselae immunoglobulin M (IgM) for serodiagnosis of cat scratch disease (CSD). IgM band patterns were examined using sera from 92 patients clinically suspected of having CSD and from 130 healthy individuals. Positive WB bands were observed in 49 (53.5%) of the 92 patients' sera. Three bands at 8--10, 31--35, and 70 kDa were regarded as relevant for B. henselae because all of the positive sera yielded at least one of the three bands, and none of the healthy control sera showed reactivity to any of them. In contrast, the positive rate of the patients' sera by conventional indirect fluorescence antibody assay (IFA) for B. henselae IgM was 28.3% (26/92) among the patients. These finding suggest that the IgM-WB assay, although cumbersome to perform, can be used for confirmatory diagnosis of CSD with no false positivity in the control sera. Purification of proteins in the specific bands may contribute to the development of an IgM-ELISA with improved specificity and sensitivity.

PMID: 29093103 [PubMed - as supplied by publisher]

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