Effects of combined magnetic fields (CMF) treatment and nano-hydroxyapatite (HA) coating on porous biphasic calcium phosphate (BCP) bone graft in rabbit spinal fusion model.

Effects of combined magnetic fields (CMF) treatment and nano-hydroxyapatite (HA) coating on porous biphasic calcium phosphate (BCP) bone graft in rabbit spinal fusion model.

Spine (Phila Pa 1976). 2017 Oct 31;:

Authors: Zhuo X, Li C, Li B, Li Z, Lv H, Huang J, Xu D, Hu JZ

Abstract
STUDY DESIGN: An animal experimental study was designed to investigate the efficacy of combined magnetic fields (CMF) treatment and nano-hydroxyapatite (HA) coating in the biphasic calcium phosphate (BCP) graft in posterolateral lumbar fusion.
OBJECTIVE: To evaluate the effects of CMF treatment and nano-HA/BCP and their combination effect in posterolateral lumbar fusion.
SUMMARY OF BACKGROUND DATA: Enhancement of artificial bone graft bioeffects could improve spinal fusion outcomes. The bone graft integration is vital in spinal fusion, nano-HA coating and CMF treatment were reported as effective methods to improve bone graft integration.
METHODS: A bilateral transverse process fusion model was performed on 32 rabbits. The CMF treatment was performed for 30 minutes per day post operation. The fusion rate, new bone formation, artificial bone graft-autologous bone fusion interface in X-ray and scanning electron microscopy, biomechanics property of fusion rate, histological fusion condition, artificial bone residual rate and immunohistochemistry assessment of BMP-2 and TGF- β1 expression were observed at 9th week after surgery.
RESULTS: CMF treatment and nanoHA coating increased the fusion rate, adjusted optical density index, intensity of binding of artificial and autologous bone, bone growth rate and bending stiffness. CMF treatment also significantly increased BMP-2 and TGF-β1 expression in fusion region while nanoHA coating significantly decreased artificial bone residual rate.
CONCLUSION: Our findings suggest that porous nano-HA/BCP graft could significantly improve spine fusion outcome with excellent bioactivity, biocompatibility and degradability and CMF treatment could significantly improve spine fusion outcome by improving bioactivity and biocompatibility of artificial bone graft in rabbit. Combination of CMF treatment with nano-HA/BCP graft could significantly increase posterolateral lumbar fusion rate in rabbit, which would be a potential strategy for spine fusion preclinical study.
LEVEL OF EVIDENCE: N/A.

PMID: 29095414 [PubMed - as supplied by publisher]

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